Protein Leverage Hypothesis Counterpoint

In 2005, Simpson and Raubenheimer postulated the ‘protein leverage hypothesis’ (PLH) to address the question of which nutrient (fat or carbohydrates) is responsible for obesity. The PLH idea is that like numerous other animal species, human macronutrient regulation minimizes variation in absolute protein intake. There is a lot of evidence to this effect (Martínez Steele E. Ultra-processed foods, protein leverage and energy intake in the USA. Public Health Nutr. 2018 Jan;21(1):114-124.).

A New Study

There’s a new study which shows that fat intake, not protein or carbohydrate intake, regulates weight gain in mice (Hu S1, et.al. Dietary Fat, but Not Protein or Carbohydrate, Regulates Energy Intake and Causes Adiposity in Mice. Cell Metab. 2018 Jul 4. pii: S1550-4131(18)30392-9).

The study exposed C57BL/6 mice to 29 different diets varying from 8.3% to 80% fat, 10% to 80% carbohydrate, 5% to 30% protein, and 5% to 30% sucrose. The study concluded that:

Mice regulate their food consumption primarily to meet an energy rather than a protein target, but this system can be over-ridden by hedonic factors linked to fat, but not sucrose, consumption.

The study has a cute graphic.

Examining the Graphic

An ad libitum fed lean mouse didn’t get leaner on higher protein. A lean mouse didn’t get fatter on high carbohydrates.

It took higher fat to make a mouse fatter. Interestingly the steps for fat were much larger than the steps for carbohydrates or protein. I can understand why protein would be limited but why did they limit carbohydrates to 30% maximum? Also, what were the other macros when protein was provided?

Fat Source

The fat source was “a mix of cocoa butter, coconut oil, menhaden oil, palm oil and sunflower oil” (‘Proof’ it’s fats, not carbs, that cause weight gain – but only in mice. Tuesday July 17 2018.) All of these are refined plant based fats with different types of fats.

Sugar and Carbohydrates

The base carbohydrate source was corn starch and maltodextrose. The sugar content (sucrose) was varied.

Protein Source

The protein source was casein (found in animal milk).

Men are not Mice

The ‘Proof’ cited above details some of the issues with the mice/men difference (‘Proof’ it’s fats, not carbs, that cause weight gain – but only in mice. Tuesday July 17 2018.).

The Peak Point

Turned out that there was a peak point in fat when more than 70% of calories from fat resulted in a drop of calories eaten. Lower levels of fat would have had higher levels of carbohydrates.

Author: Doug

I'm an Engineer who is also a science geek. I was pre-diabetic in 1996 and became a diabetic in 2003. I decided to figure out how to hack my diabetes and in 2016 found the ketogetic diet which reversed my diabetes.

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